SPARC Protein (AA 18-297) (His-IF2DI Tag)
Quick Overview for SPARC Protein (AA 18-297) (His-IF2DI Tag) (ABIN7125003)
Target
See all SPARC ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 18-297
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Purification tag / Conjugate
- This SPARC protein is labelled with His-IF2DI Tag.
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Purpose
- Recombinant Human SPARC
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Sterility
- 0.2 μm filtered
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Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) (Active) protein (His tag)
SPARC Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) protein (GST tag)SPARC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) protein (His tag)SPARC Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 99-330) protein (Fc Tag)SPARC Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full length) protein (GST tag)SPARC Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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Expiry Date
- 12 months
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- SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))
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Alternative Name
- SPARC
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Background
- AA517111, Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40, BM40, Cysteine rich protein, hm:zeh0062, MGC128090, OI17, ON, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and cysteine rich, Secreted protein acidic and rich in cysteine
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Molecular Weight
- 30.7 kDa
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UniProt
- P09486
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Pathways
- Autophagy
Target
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