UBE2N Protein (AA 2-152) (His tag)

Catalog No. ABIN7125625

Recombinant UBE2N protein expressed in Escherichia coli (E. coli).

Quick Overview for UBE2N Protein (AA 2-152) (His tag) (ABIN7125625)

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 95 % as determined by SDS-PAGE

Product Details

Protein Characteristics: AA 2-152

Purification tag / Conjugate: This UBE2N protein is labelled with His tag.

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution

Expiry Date: 12 months

Target Details

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Alternative Name: Ube2N

Background: Bendless like ubiquitin conjugating enzyme, Bendless-like ubiquitin-conjugating enzyme, BLU, EC 6.3.2.19, Epididymis secretory protein Li 71, HEL-S-71, Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds

Molecular Weight: 16.5 kDa

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway