Vinculin Protein (VCL) (AA 2-235) (His tag)

Catalog No. ABIN7125718

Recombinant Vinculin protein expressed in Escherichia coli (E. coli).

Quick Overview for Vinculin Protein (VCL) (AA 2-235) (His tag) (ABIN7125718)

Target: Vinculin (VCL)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 95 % as determined by SDS-PAGE

Product Details

Protein Characteristics: AA 2-235

Purification tag / Conjugate: This Vinculin protein is labelled with His tag.

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution

Expiry Date: 12 months

Target Details

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, OTTHUMP00000019861, OTTHUMP00000019862, VCL, VINC, VINC_HUMAN, Vinculin

Molecular Weight: 25.6 kDa

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2