CD19 Protein (His tag)

Catalog No. ABIN7125883

This Recombinant CD19 protein is expressed in HEK-293 Cells.

Quick Overview for CD19 Protein (His tag) (ABIN7125883)

Target: CD19 (CD19 Molecule (CD19))

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Purity: >90 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD19 protein is labelled with His tag.

Purpose: Rat CD19 Protein, His Tag

Sequence: Arg 19 - Gly 287

Characteristics: Rat CD19, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 19 - Gly 287 (Accession # F1LNH2-1).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 31.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: Synonyms: CD19,B4,CVID3,MGC12802,
B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I  membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.

Molecular Weight: 31.7 kDa

NCBI Accession: NP_001013255

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway