Cas12a Nuclease (Cpf1) Protein

Catalog No. ABIN7125967

This Recombinant Cas12a Nuclease protein is expressed in Escherichia coli (E. coli).

Quick Overview for Cas12a Nuclease (Cpf1) Protein (ABIN7125967)

Target: Cas12a Nuclease (Cpf1)

Protein Type: Recombinant

Origin: Bacteria (Lachnospiraceae)

Source: Escherichia coli (E. coli)

Purity: >95 % as determined by SDS-PAGE.

Grade: MALS verified

Product Details

Purpose: NLS-Cas12a Nuclease (MALS verified)

Sequence: Ser 2 - His 1228

Characteristics: NLS-Cas12a Nuclease is expressed from E.coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).

Endotoxin Level: Less than 0.01 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 147.6 kDa. The protein migrates as kDa under reducing (R) condition . This protein has two NLS, which leads to transport to the nucleus efficiently. It is ideal for in vitro and in vivo reactions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM NaAC, 500 mM NaCl, 0.1 mM EDTA, 0.1 mM TCEP, pH 6.0

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: Cas12a Nuclease (Cpf1)

Alternative Name: NLS-Cas12a Nuclease

Background: Synonyms: CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1, cas12a, Cpf1,
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated endonuclease from Lachnospiraceae bacterium,Cas12a, also known as Cpf1, is a 1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system , an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing. CRISPR/Cas12a belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector. Cas12a has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crRNA, inducing a staggered double-strand break and potential for both RNA processing and DNA nuclease activity. In addition, Cas12a is able to process more structured pre-CRISPR/RNA(crRNA) molecules into mature crRNAs which allows the possibility to use both mature or pre-crRNA for genome editing purposes. All these features make the CRISPR/Cas12a system a valuable genome-engineering tool. CRISPR-Cas12a has been successfully used to edit genomes in many mammalians cells and plants.

Molecular Weight: 147.6 kDa