ApoE3 Protein (R154S) (His-Avi Tag,Biotin)

Catalog No. ABIN7199215

Recombinant ApoE3 protein expressed in HEK-293 Cells.

Quick Overview for ApoE3 Protein (R154S) (His-Avi Tag,Biotin) (ABIN7199215)

Target: ApoE3 (Apolipoprotein E3 (ApoE3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >90 % as determined by SDS-PAGE.

Grade: MALS verified

Product Details

Protein Characteristics: R154S

Purification tag / Conjugate: This ApoE3 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human Apolipoprotein E / APOE3 (R154S) Protein, His,Avitag™ (MALS verified)

Sequence: Lys 19 - His 317

Characteristics: Biotinylated Human APOE3 (R154S), His,Avitag is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (Accession # P02649-1 (R154S).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 37.7 kDa. The protein migrates as 39-44 kDa under reducing (R) condition due to glycosylation.

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, 5 mM CHAPS, 2 mM DTT, pH 7.4

Storage: -20 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Target Details

Target: ApoE3 (Apolipoprotein E3 (ApoE3))

Alternative Name: Apolipoprotein E / APOE3

Background: Synonyms: Apolipoprotein E,APOE,Apo-E,
Description: APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles.

Molecular Weight: 37.7 kDa

Pathways: Regulation of Cell Size, Lipid Metabolism