Quick Overview for CD73 Protein (His tag) (ABIN7321272)
Target
See all CD73 (NT5E) ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This CD73 protein is labelled with His tag.
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Purpose
- Recombinant Rat CD73/NT5E Protein (His Tag)
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Sequence
- Trp29-Lys549
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Characteristics
- Recombinant Rat 5'-Nucleotidase/CD73 is produced by our Mammalian expression system and the target gene encoding Trp29-Lys549 is expressed with a 6His tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-549) (Active) protein (His tag)
NT5E Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-554) (Active) protein (His tag)NT5E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) (Active) protein (hFc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (Fc Tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (His tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) protein (Fc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) (Active) protein (His tag,AVI tag,Biotin)NT5E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 28-264) protein (GST tag)NT5E Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
5'-Nucleotidase, Ecto (CD73) (NT5E) protein (His tag)NT5E Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 34-214) protein (His tag)NT5E Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 120 mM NaCl, 4 mM CaCl2, 20 % Glycerol, pH 7.5.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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Alternative Name
- CD73/NT5E
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Background
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Background: CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ' -Nucleotidase activity of CD73 converts extracellular nucleoside-5 ' -monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Synonym: CD73, E5NT,Nucleotidase, NT5E,ecto-5'-nucleotidase,5'-Nucleotidase
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Molecular Weight
- 59 kDa
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UniProt
- P21588
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Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
Target
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