ACVR2B Protein (His tag)

Catalog No. ABIN7320385

This Recombinant ACVR2B protein is produced in HEK-293 Cells.

Quick Overview for ACVR2B Protein (His tag) (ABIN7320385)

Target: ACVR2B (Activin A Receptor, Type IIB (ACVR2B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 94 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This ACVR2B protein is labelled with His tag.

Purpose: Recombinant Mouse Activin Receptor 2B/ACVR2B Protein (His Tag)(Active)

Sequence: Met 1-Thr 134

Characteristics: A DNA sequence encoding the extracellular domain of mouse ACTRIIB (NP_031423.1) (Met 1-Thr 134) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA. Immobilized human ACVR2B at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His, The EC50 of biotinylated mouse INHBA-His is 0.161 μg/mL.2. Measured by its binding ability in a functional ELISA. Immobilized mouse INHBA-his at 10 μg/mL (100 μl/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is 0.39 μg/mL.3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2-0.8 μg/mL in the presence of 10 ng/mL recombinant Activin A.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ACVR2B (Activin A Receptor, Type IIB (ACVR2B))

Alternative Name: Activin Receptor 2B/ACVR2B

Background:

Background: ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.

Synonym: 4930516B21Rik,ActRIIB

Molecular Weight: 14.8 kDa

NCBI Accession: NP_031423

Pathways: Hormone Transport, Cancer Immune Checkpoints