Activin RIIA / ACVR2A (Active) protein (His tag)

Catalog No. ABIN7194139

This Recombinant Activin RIIA / ACVR2A protein is expressed in HEK-293 Cells.

Quick Overview for Activin RIIA / ACVR2A (Active) protein (His tag) (ABIN7194139)

Target: Activin RIIA / ACVR2A

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 97 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Mouse Activin RIIA/ACVR2A Protein (His Tag)(Active)

Sequence: Met 1-Pro 134

Characteristics: A DNA sequence encoding the extracellular domain of mouse ACVR2A (NP_031422.3) (Met 1-Pro 134) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED50 for this effect is typically 0.6-3 μg/mL in the presence of 10 ng/ml Recombinant Human Activin A.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Activin RIIA / ACVR2A

Alternative Name: Activin RIIA/ACVR2A

Background:

Background: ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98 % identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.

Synonym: ActrIIa,Acvr2,TactrII

Molecular Weight: 14.8 kDa

NCBI Accession: NP_031422