CD13 Protein (His tag)

Catalog No. ABIN7194190

This Recombinant CD13 protein is expressed in HEK-293 Cells.

Quick Overview for CD13 Protein (His tag) (ABIN7194190)

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 97 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CD13 protein is labelled with His tag.

Purpose: Recombinant Mouse ANPEP/CD13 Protein (His Tag)(Active)

Sequence: Lys 66-Ser 966

Characteristics: A DNA sequence encoding the metalloprotease domain (Lys 66-Ser 966) of mouse ANPEP (NP_032512.2) was expressed with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >800 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: ANPEP/CD13

Background:

Background: Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.

Synonym: AP-M,AP-N,Apn,Cd13,P150

Molecular Weight: 104 kDa

NCBI Accession: NP_032512

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones