ASF1A Protein (T7 tag,His tag)
Quick Overview for ASF1A Protein (T7 tag,His tag) (ABIN7318587)
Target
See all ASF1A ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This ASF1A protein is labelled with T7 tag,His tag.
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Purpose
- Recombinant Human ASF1A Protein (His&T7 Tag)
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Sequence
- Met 1-Met204
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Characteristics
- Recombinant Human Histone Chaperone ASF1A is produced by our E.coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A) (AA 1-204) protein (His tag)
custom-made ASF1A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A) (AA 1-204) protein (His tag)custom-made ASF1A Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A) (AA 1-204) protein (GST tag)ASF1A Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A) (AA 1-204) protein (His tag)ASF1A Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 150 mM NaCl, pH 8.0.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- ASF1A (ASF1 Anti-Silencing Function 1 Homolog A (S. Cerevisiae) (ASF1A))
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Alternative Name
- ASF1A
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Background
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Background: Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many cells and tissues, interacting with histones H3 and H4. ASF1A cooperates with Chromatin Assembly Factor 1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. In addition, ASF1A is necessary for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Synonym: Histone Chaperone ASF1A, Anti-Silencing Function Protein 1 Homolog A, hAsf1, hAsf1a, CCG1-Interacting Factor A, CIA, hCIA, ASF1A
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Molecular Weight
- 25.4 kDa
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UniProt
- Q9Y294
Target
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