ATG5 Protein
Quick Overview for ATG5 Protein (ABIN7318187)
Target
See all ATG5 ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Recombinant Human ATG5 Protein
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Sequence
- Met 1-Asp275
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Characteristics
- Recombinant Human Autophagy Related 5 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Asp275 is expressed.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (His tag)
custom-made ATG5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (His tag)custom-made ATG5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATG5 Autophagy Related 5 (ATG5) (AA 138-275) protein (His-IF2DI Tag)ATG5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (His-IF2DI Tag)ATG5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (GST tag)ATG5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (His tag)ATG5 Origin: Human, Mouse Host: Escherichia coli (E. coli) Recombinant ~95 % ELISA, WB, MS, Neut
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris,0.2M NaCl,1 mM DTT,40 % glycerol, pH 8.0 .
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- ATG5 (ATG5 Autophagy Related 5 (ATG5))
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Alternative Name
- ATG5
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Background
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Background: ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.
Synonym: Autophagy protein 5, APG5-like, Apoptosis-specific protein, APG5L, ASP, ATG5,
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Molecular Weight
- 32.4 kDa
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UniProt
- Q9H1Y0
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Pathways
- Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy
Target
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