ACLY Protein (His tag)
Quick Overview for ACLY Protein (His tag) (ABIN7194322)
Target
See all ACLY ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This ACLY protein is labelled with His tag.
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Purpose
- Recombinant Human ACLY/acly/ATP citrate lyase Protein (His Tag)
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Sequence
- Met 1-Met 1101
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Characteristics
- A DNA sequence encoding the human ACLY (P53396) (Met 1-Met 1101) was expressed, with a polyhistidine tag at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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ATP Citrate Lyase (ACLY) (AA 1-1091) protein (His tag)
custom-made ACLY Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATP Citrate Lyase (ACLY) (AA 1-1101) protein (His tag)custom-made ACLY Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATP Citrate Lyase (ACLY) (AA 4-265) protein (His tag)ACLY Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
ATP Citrate Lyase (ACLY) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ACLY Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- ACLY (ATP Citrate Lyase (ACLY))
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Alternative Name
- ACLY/acly/ATP citrate lyase
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Background
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Background: ATP citrate lyase, also known as Acly or Acl, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is composed of two polymer chains which are polypeptides in human. ATP citrate lyase is responsible for catalyzing the conversion of citrate and CoA into acetyl-CoA and oxaloacetate, along with the hydrolysis of ATP. A definitive role for ATP citrate lyase in tumorigenesis has emerged from ATP citrate lyase RNAi and chemical inhibitor studies, showing that ATP citrate lyase inhibition limits tumor cell proliferation and survival and induces differentiation in vitro. In vivo, it reduces tumor growth leading to a cytostatic effect and induces differentiation.
Synonym: ACL;ATPCL;CLATP
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Molecular Weight
- 123 kDa
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UniProt
- P53396
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Pathways
- Warburg Effect
Target
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