BID Protein

Catalog No. ABIN7317857

This Recombinant BID protein is produced in Escherichia coli (E. coli).

Quick Overview for BID Protein (ABIN7317857)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human BID Protein (Active)

Sequence: Met 1-Asp 195

Characteristics: A DNA sequence encoding the human BID isoform 1 (P55957-1) (Met 1-Asp 195) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA.2. Immobilized human BID at 10 μg/mL (100 μl/well) can bind  biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.1 ng/mL.3. Immobilized human BID at 10 μg/mL (100 μl/well) can bind  biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 40 mM Tris, 150 mM NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background:

Background: The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Synonym: BH3-Interacting Domain Death Agonist, p22 BID, BID

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity