Quick Overview for BRD4 Protein (ABIN7319875)
Target
See all BRD4 ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Recombinant Human BRD4 (N-10His-Flag)
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Sequence
- Glu49-Glu460
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Characteristics
- Recombinant Human Bromodomain-containing protein 4 is produced by our E.coli expression system and the target gene encoding Glu49-Glu460 is expressed with a 10His, Flag tag at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Bromodomain Containing 4 (BRD4) (AA 1-1400) protein (His tag)
custom-made BRD4 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Bromodomain Containing 4 (BRD4) (AA 1-1362) protein (His tag)custom-made BRD4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Bromodomain Containing 4 (BRD4) (AA 49-170) protein (His tag)BRD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Bromodomain Containing 4 (BRD4) (AA 1-1362) protein (Fc Tag)BRD4 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Bromodomain Containing 4 (BRD4) (AA 44-168) protein (His tag,DYKDDDDK Tag)BRD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Bind, ScA
Bromodomain Containing 4 (BRD4) (AA 333-460) protein (His tag,DYKDDDDK Tag)BRD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Bind, ScA
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 1 mM DTT, 10 % Glycerol, pH 7.5.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- BRD4 (Bromodomain Containing 4 (BRD4))
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Alternative Name
- BRD4
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Background
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Background: Bromodomain-containing protein 4 (BRD4) is a member of the BET class chromatin reader proteins that bind acetylated histones and play a key role in transcriptional regulation and transmission of epigenetic memory. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. BRD bromodomains serve as recognition motifs for acetylated lysine residues on histones, while the NET domain may function by promoting phosphorylation of the C-terminal domain (CTD) of RNA Polymerase II. Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. BRD4 is a potential therapeutic target in many diseases including breast cancer, AML, multiple myeloma, colon cancer and others.
Synonym: HUNK1, HUNK1bromodomain-containing protein 4, HUNKI, MCAP
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Molecular Weight
- 49 kDa
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UniProt
- O60885
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Pathways
- Chromatin Binding, SARS-CoV-2 Protein Interactome
Target
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