Cadherin 12 Protein (His tag)

Catalog No. ABIN7317919

This Recombinant Cadherin 12 protein is expressed in HEK-293 Cells.

Quick Overview for Cadherin 12 Protein (His tag) (ABIN7317919)

Target: Cadherin 12

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Cadherin 12 protein is labelled with His tag.

Purpose: Recombinant Human Cadherin-12/CDH12 Protein (His Tag)

Sequence: Met 1-Ala 605

Characteristics: A DNA sequence encoding the pro form of human CDH12 (NP_001783.2) extracellular domain (Met 1-Ala 605) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM sodium citrate, 50 mM NaCl, 2 mM CaCl2, pH 6.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Cadherin 12

Alternative Name: Cadherin-12/CDH12

Background:

Background: Classic Cadherins represent a family of calcium-dependent homophilic cell-cell adhesion molecules. They confer strong adhesiveness to animal cells when they are anchored to the actin cytoskeleton via their cytoplasmic binding partners, catenins. The cadherin/catenin adhesion system plays key roles in the morphogenesis and function of the vertebrate and invertebrate nervous systems. Furthermore, this system is involved in synaptic plasticity. Recent studies on the role of individual cadherin subtypes at synapses indicate that individual cadherin subtypes play their own unique role to regulate synaptic activities. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-12 also known as CDH12, is a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherin-12 appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.

Synonym: CDHB

Molecular Weight: 66 kDa

NCBI Accession: NP_001783