CA12 Protein (His tag)

Catalog No. ABIN7194556

This Recombinant CA12 protein is expressed in HEK-293 Cells.

Quick Overview for CA12 Protein (His tag) (ABIN7194556)

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CA12 protein is labelled with His tag.

Purpose: Recombinant Mouse Carbonic Anhydrase XII/CA12 Protein (His Tag)(Active)

Sequence: Met 1-Ser 301

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Ser 301) of mouse CA12 (NP_848483.2) precursor was expressed with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its esterase activity. The specific activity is >50 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.4 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Alternative Name: Carbonic Anhydrase XII/CA12

Background:

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I  membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

Synonym: Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; CA12; Carbonate dehydratase XII; CAXII;Car12

Molecular Weight: 32.8 kDa

NCBI Accession: NP_848483