CA2 Protein (His tag)

Catalog No. ABIN7194562

This Recombinant CA2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CA2 Protein (His tag) (ABIN7194562)

Target: CA2 (Carbonic Anhydrase II (CA2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 96 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CA2 protein is labelled with His tag.

Purpose: Recombinant Mouse Carbonic Anhydrase II/CA2 Protein (His Tag)(Active)

Sequence: Met 1-Lys 260

Characteristics: A DNA sequence encoding the mouse CA2 (AAH55291.1) (Met 1-Lys 260) was expressed, with a C-terminal polyhistidine tag.

Biological Activity Comment: Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase II/CA2

Background:

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

Synonym: AI131712;Ca2;CAII;Car-2;Ltw-5;Lvtw-5

Molecular Weight: 30.4 kDa