CA9 Protein (His tag)

Catalog No. ABIN7194573

This Recombinant CA9 protein is produced in HEK-293 Cells.

Quick Overview for CA9 Protein (His tag) (ABIN7194573)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CA9 protein is labelled with His tag.

Purpose: Recombinant Mouse Carbonic Anhydrase IX/CA9 Protein (His Tag)(Active)

Sequence: Met 1-Asp 390

Characteristics: A DNA sequence encoding the extracellular domain of mouse CAR9 (NP_647466.2) (Met 1-Asp 390) was expressed, with a C-terminal polyhistidine tag.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: Carbonic Anhydrase IX/CA9

Background:

Background: Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: RP23-195K8.9, CAIX, Ca9, MN/CA9

Molecular Weight: 40.3 kDa

NCBI Accession: NP_647466