CA9 Protein (AA 38-402) (His tag)
-
- Target See all CA9 Proteins
- CA9 (Carbonic Anhydrase IX (CA9))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 38-402
-
Origin
- Cynomolgus
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CA9 protein is labelled with His tag.
- Purpose
- Cynomolgus CA9/Carbonic Anhydrase IX Protein
- Sequence
- Gln38-Asp402
- Characteristics
- Recombinant Cynomolgus CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln38-Asp402.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus CA9, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA. See testing image for detail.
-
Carbonic Anhydrase IX (CA9) (AA 38-414) protein (His-Avi Tag)
CA9 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase IX (CA9) (AA 32-390) protein (His tag)CA9 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase IX (CA9) (AA 1-459) protein (GST tag)CA9 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase IX (CA9) (AA 38-153) protein (His-IF2DI Tag)CA9 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Carbonic Anhydrase IX (CA9) protein (His tag)CA9 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Carbonic Anhydrase IX (CA9) (Active) protein (His tag)CA9 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Carbonic Anhydrase IX (CA9) protein (His tag,Biotin)CA9 Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Carbonic Anhydrase IX (CA9) (AA 38-414) protein (His tag)Crystallography grade CA9 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase IX (CA9) (AA 32-390) protein (His tag)Crystallography grade CA9 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase IX (CA9) (AA 38-459) protein (rho-1D4 tag)Crystallography grade CA9 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- CA9 (Carbonic Anhydrase IX (CA9))
- Alternative Name
- CA9 (CA9 Products)
- Synonyms
- CA9 Protein, CAIX Protein, Ca9 Protein, MN/CA9 Protein, MN Protein, carbonic anhydrase 9 Protein, CA9 Protein, CAH9 Protein, Car9 Protein
- Background
- CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.
- Molecular Weight
- 41.03 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A2K5VQG9
-
