CMBL Protein (His tag)
Quick Overview for CMBL Protein (His tag) (ABIN7317566)
Target
See all CMBL ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This CMBL protein is labelled with His tag.
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Purpose
- Recombinant Human CMBL Protein (His Tag)
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Sequence
- Met 1-Met 245
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Characteristics
- A DNA sequence encoding the human CMBL (Q96DG6) (Met 1-Met 245) was expressed, with a N-terminal polyhistidine tag.
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Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (His tag)
CMBL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (GST tag)CMBL Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Carboxymethylenebutenolidase Homolog (CMBL) protein (Myc-DYKDDDDK Tag)CMBL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from sterile 20 mM Tris, 0.1 % Brij35, pH 8.0
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CMBL (Carboxymethylenebutenolidase Homolog (CMBL))
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Alternative Name
- CMBL
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Background
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Background: Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol- lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of hydrolases. It maily acts on carboxylic ester bonds. CMBL is a human homolog of Pseudomonas dienelactone hydrolase involved in the bacterial halocatechol degradation pathway. The ubiquitous expression of human CMBL gene transcript in various tissues was observed. CMBL was demonstrated to be the primary olmesartan medoxomil (OM) bioactivating enzyme in the liver and intestine. The recombinant human CMBL expressed in mammalian cells was clearly shown to activate OM. The recombinant CMBL also converted other prodrugs having the same ester structure as OM, faropenem medoxomil and lenampicillin, to their active metabolites. CMBL exhibited a unique sensitivity to chemical inhibitors, thus, being distinguishable from other known esterases.
Synonym: JS-1
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Molecular Weight
- 30 kDa
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UniProt
- Q96DG6
Target
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