CD180 Protein (His tag)

Catalog No. ABIN7320258

Product Details

Target: CD180 (CD180 Molecule (CD180))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This CD180 protein is labelled with His tag.

Purpose: Recombinant Mouse CD180/RP105/LY64 Protein (His Tag)(Active)

Sequence: Met 1-Ser 626

Characteristics: A DNA sequence encoding the mouse CD180 (NP_032559.2) extracellular domain (Met 1-Ser 626) was expressed, fused with a polyhistidine tag at the C-terminus.

Purity: > 92 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized mouse CD180 at 2 μg/ml (100 μl/well) can bind biotinylated mouse MD-1 with a linear ranger of 6.25-50 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD180 (CD180 Molecule (CD180))

Alternative Name: CD180/RP105/LY64 (CD180 Products)

Synonyms: Ly78 Protein, CD180 Protein, LY64 Protein, RP105 Protein, F630107B15 Protein, CD180 molecule Protein, CD180 antigen Protein, Cd180 Protein, CD180 Protein

Background:

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD180, also known as RP105, is a B-cell surface molecule belonging to the family of pathogen receptors, Toll-like receptors (TLR). CD180 has an extracellular leucine-rich repeats and a short cytoplasmic tail. CD180 / RP105 interact with an extracellular molecule named MD1 and then together form the cell surface receptor complex RP105 / MD1 which induces B-cell activation in humans and mice, leading to proliferation and up-regulation of a costimulatory molecule, B7.2 / CD86. CD180 / RP105 also has a role in LPS response because B cells lacking RP105 show hyporesponsiveness to LPS.

Synonym: F630107B15,Ly78,RP105

Molecular Weight: 69.4 kDa

NCBI Accession: NP_032559

Pathways: Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades