CD40 Ligand Protein (CD40LG) (His tag)

Catalog No. ABIN7320848

This Recombinant CD40 Ligand protein is produced in Human Cells.

Quick Overview for CD40 Ligand Protein (CD40LG) (His tag) (ABIN7320848)

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD40 Ligand protein is labelled with His tag.

Purpose: Recombinant Mouse CD40LG/TNFSF5 Protein (His Tag)(Active)

Sequence: Met112-Leu260

Characteristics: Recombinant Mouse CD40L is produced by our Mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human CD40-Fc(Cat: PKSM041225) at 2μg/ml(100 μl/well) can bind Human CD40LG-His. The ED50 of Human CD40LG-His(Cat: PKSH033600)) is 13.84ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40LG/TNFSF5

Background:

Background: CD40 Ligand, also known as TNFSF5, CD154, is a type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells,macrophages, monocytes, platelets, endothelial cells, and epithelial cells. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.

Synonym: CD40 Ligand, CD40LG,HIGM1, T-B cell-activating molecule, T-BAM, TNFSF5, tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5

Molecular Weight: 18.8 kDa

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints