CST7 Protein (Fc Tag)

Catalog No. ABIN7195337

Recombinant CST7 protein expressed in HEK-293 Cells.

Quick Overview for CST7 Protein (Fc Tag) (ABIN7195337)

Target: CST7 (Cystatin F (Leukocystatin) (CST7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 93 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This CST7 protein is labelled with Fc Tag.

Purpose: Recombinant Mouse Cystatin 7/CST7 Protein (Fc Tag)(Active)

Sequence: Met1-Gln144

Characteristics: A DNA sequence encoding the mouse CST7 (O89098) (Met1-Gln144) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 is < 6 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM Tris, 0.15M NaCl, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CST7 (Cystatin F (Leukocystatin) (CST7))

Alternative Name: Cystatin 7/CST7

Background:

Background: The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

Synonym: Cystatin-F, Cystatin-like Metastasis-Associated Protein, Leukocystatin, CMAP, Cystatin-7, Cst7

Molecular Weight: 41.4 kDa

UniProt: O89098