DPP4 Protein

Catalog No. ABIN7195427

Recombinant DPP4 protein expressed in HEK-293 Cells.

Quick Overview for DPP4 Protein (ABIN7195427)

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 70 % as determined by SDS-PAGE

Product Details

Purpose: Recombinant Human DPP4/CD26 Protein(Active)

Sequence: Asn29-Pro766

Characteristics: The native mature form of human DPPIV (NP_001926.2) extracellular domain (Asn 29-Pro 766) was expressed and purified.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: 1. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 2,500 pmoles/min/μg. 2. Using the Octet RED System, the affinity constant (Kd) of Recombinant Human DPP4/CD26 Protein(Active)(Cat: PKSH033811) bound to Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag) (Cat: PKSV030236) was 33 nM. 3. Using the Octet RED System, the affinity constant (Kd) of Recombinant Human DPP4/CD26 Protein(Active)(Cat: PKSH033811) bound to Recombinant HCoV-HKU1 (Isolate N1) S1 Protein (His Tag) (Cat: PKSV030109) was 12 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 100 mM NaCl, 50 mM Tris, pH 7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Alternative Name: DPP4/CD26

Background:

Background: Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.

Synonym: ADABP Protein, Human, ADCP2 Protein, Human, CD26 Protein, Human, DPPIV Protein, Human, TP103 Protein, Human

Molecular Weight: 85.4kDa

NCBI Accession: NP_001926

Pathways: Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity