Ephrin A1 Protein (EFNA1) (His tag)

Catalog No. ABIN7318434

This Recombinant Ephrin A1 protein is produced in Human Cells.

Quick Overview for Ephrin A1 Protein (EFNA1) (His tag) (ABIN7318434)

Target: Ephrin A1 (EFNA1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Ephrin A1 protein is labelled with His tag.

Purpose: Recombinant Human Ephrin-A1/EFNA1 Protein (His Tag)(Active)

Sequence: Asp19-Ser182

Characteristics: Recombinant Human Ephrin-A1 is produced by our Mammalian expression system and the target gene encoding Asp19-Ser182 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human Ephrin-A1-His at 10μg/ml(100 μl/well) can bind Human EphA2-Fc(Cat: PKSH032384). The ED50 of Human Ephrin-A1-His is 12.43ug/ml .

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Ephrin A1 (EFNA1)

Alternative Name: Ephrin-A1/EFNA1

Background:

Background: Ephrin-A1 is a member of the A-type ephrin family of cell surface proteins that function as ligands for the A-type Eph receptor tyrosine kinase family. Ephrin-A1 can be induced by TNF and IL1B. Its expression levels can be down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells. Soluble Ephrin-A1 is necessary for the transformation of HeLa and SK-BR3 cells and participates in the relocalization of EPHA2 away from sites of cell-cell contact during transformation. Ephrin-A1 plays an important role in angiogenesis and tumor neovascularization.

Synonym: Ephrin-A1, EPH-Related Receptor Tyrosine Kinase Ligand 1, LERK-1, Immediate Early Response Protein B61, Tumor Necrosis Factor Alpha-Induced Protein 4, TNF Alpha-Induced Protein 4, EFNA1, EPLG1, LERK1, TNFAIP4

Molecular Weight: 20.4 kDa

UniProt: P20827

Pathways: RTK Signaling