Ethanolaminephosphotransferase 1 (SELENOI) protein (GST tag)

Catalog No. ABIN7318453

Recombinant Ethanolaminephosphotransferase 1 protein expressed in Escherichia coli (E. coli).

Quick Overview for Ethanolaminephosphotransferase 1 (SELENOI) protein (GST tag) (ABIN7318453)

Target: Ethanolaminephosphotransferase 1 (SELENOI)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: GST tag

Purpose: Recombinant Human SELENOI/EPT1 Protein (GST Tag)

Sequence: Met 1-Pro50

Characteristics: Recombinant Human Ethanolaminephosphotransferase 1 is produced by our E.coli expression system and the target gene encoding Met1-Pro50 is expressed with a GST tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: Ethanolaminephosphotransferase 1 (SELENOI)

Alternative Name: SELENOI/EPT1

Background:

Background: Ethanolaminephosphotransferase 1 (EPT1) is an enzyme that belongs to the CDP-Alcohol Phosphatidyltransferase Class-I Family. EPT1 is a Selenoprotein, which contains a Selenocysteine (Sec) residue at its active site. The Selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. EPT1 catalyzes Phosphatidylethanolamine biosynthesis from CDP-Ethanolamine. It plays a central role in the formation and maintenance of vesicular membranes. EPT1 is involved in the formation of Phosphatidylethanolamine via the 'Kennedy' pathway.

Synonym: Ethanolaminephosphotransferase 1, hEPT1, Selenoprotein I, SelI, EPT1, KIAA1724, SELI

Molecular Weight: 32.3 kDa

UniProt: Q9C0D9