Extracellular Serine/threonine Protein Kinase FAM20C (FAM20C) protein (His tag)

Catalog No. ABIN7317159

This Recombinant Extracellular Serine/threonine Protein Kinase FAM20C protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for Extracellular Serine/threonine Protein Kinase FAM20C (FAM20C) protein (His tag) (ABIN7317159)

Target: Extracellular Serine/threonine Protein Kinase FAM20C (FAM20C)

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Human FAM20C/DMP4 Protein (His Tag)

Sequence: Met 1-Arg584

Characteristics: A DNA sequence encoding the human FAM20C (NP_064608.2) (Met1-Arg584) was expressed with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Extracellular Serine/threonine Protein Kinase FAM20C (FAM20C)

Alternative Name: FAM20C/DMP4

Background:

Background: Fam20C is a protein kinase dedicated to the phosphorylation of extracellular proteins, including caseins, and is localizes within the Golgi apparatus and also is secreted. It phosphorylates the caseins as well as several other secreted proteins such as AMELX, AMTN, ENAM, SPP1 and the small integrin-binding ligand N-linked glycoproteins SIBLINGs implicated in biomineralization. Consequently, mutations in Fam20C cause an osteosclerotic bone dysplasia in humans known as Raine syndrome and hypophosphatemic rickets. The discovery of the function of Fam20C has solved a 130-year-old mystery of the identity of the kinases that phosphorylate caseins and has shed light on several human disorders of biomineralization. In addition to its role in biomineralization, Fam20C also plays a role in lipid homeostasis, wound healing and cell migration and adhesion.

Synonym: DMP-4,DMP4,GEF-CK,RNS

Molecular Weight: 65.1 kDa

NCBI Accession: NP_064608