FCGR2B Protein (His tag)

Catalog No. ABIN7195694

Recombinant FCGR2B protein expressed in HEK-293 Cells.

Quick Overview for FCGR2B Protein (His tag) (ABIN7195694)

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 97 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This FCGR2B protein is labelled with His tag.

Purpose: Recombinant Rat CD32b/FCGR2B Protein (His Tag)(Active)

Sequence: Met 1-Pro 212

Characteristics: A DNA sequence encoding the rat FCGR2B (Q63203-1) extracellular domain (Met 1-Pro 212) was expressed, fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized human rat FCGR2B-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.3-0.8 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Alternative Name: CD32b/FCGR2B

Background:

Background: FcγRIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of FcγRIIA, FcγRIIB, and FcγRIIC proteins that share 94-99 % sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. FcγRII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. FcγRIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of FcγRIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of FcγRIIB on dendritic cells inhibits maturation and blocks cell activation. FcγRIIB may also be a target for monoclonal antibody therapy for malignancies. FcγRIIB plays an important negative-regulating role through modulating the signals from activation receptors.

Synonym: FCRII,Fcgr2

Molecular Weight: 21.9 kDa

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling