FCGR3A Protein (His tag,AVI tag)

Catalog No. ABIN7195704

This Recombinant FCGR3A protein is produced in HEK-293 Cells.

Quick Overview for FCGR3A Protein (His tag,AVI tag) (ABIN7195704)

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This FCGR3A protein is labelled with His tag,AVI tag.

Purpose: Recombinant Human CD16a/FCGR3A Protein (176 Val, His&AVI Tag)(Active)

Sequence: Met 1-Gln 208

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (AAH17865.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. It is identical to FCGR3A158F/V in the reference.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human CD16a-AVI-His (V158) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.08-0.18 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Alternative Name: CD16a/FCGR3A

Background:

Background: The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

Synonym: Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A, CD16a Antigen, Fc-Gamma RIII-Alpha, Fc-Gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc Receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3,CD16,CD16A

Molecular Weight: 25.6 kDa