FCGR3A Protein (AA 17-208) (His-Avi Tag,Biotin)

Catalog No. ABIN7536872

Product Details

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Protein Type: Recombinant

Protein Characteristics: AA 17-208

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FCGR3A protein is labelled with His-Avi Tag,Biotin.

Application: SDS-PAGE (SDS), Surface Plasmon Resonance (SPR), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC)

Purpose: Biotinylated human Fc gamma RIIIa / CD16a (176F) protein

Sequence: GMRTEDLPKA VVFLEPQWYR VLEKDSVTLK CQGAYSPEDN STQWFHNESL ISSQASSYFI DAATVDDSGE YRCQTNLSTL SDPVQLEVHI GWLLLQAPRW VFKEEDPIHL RCHSWKNTAL HKVTYLQNGK GRKYFHHNSD FYIPKATLKD SGSYFCRGLF GSKNVSSETV NITITQGLAV STISSFFPPG YQGGGLNDIF EAQKIEWHEG GGENLYFQSG GHHHHHHHHH H

Specificity: IgG

Characteristics: The sequence of the extracellular domain of human CD16a (Gly 17-Gln 208) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Allotype: 176F - the low affinity polymorphic variant for CD16a.

Purification: Nickel and SEC

Purity: > 95 % by SEC-HPLC

Endotoxin Level: <1.0 EU per mg

Biological Activity Comment: Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RIIIa / CD16a (176F) protein, directly immobilized on a CM5 chip, can bind to anti-HER2 human IgG1 (trastuzumab) with an affinity constant (KD) of 610 nM.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biotin to protein ratio is confirmed as 0.7-1.0 by the HABA assay. Product has been site-specifically biotinylated using the AVI tag technology, where the lysine residue within the tag is enzymatically labeled with biotin.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.

Concentration: 1 mg/mL

Buffer: PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)

Preservative: Without preservative

Storage: RT,4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.

Expiry Date: 12 months

Target Details

Target: FCGR3A (Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A))

Alternative Name: CD16a (FCGR3A Products)

Synonyms: CD16 Protein, CD16A Protein, FCG3 Protein, FCGR3 Protein, FCGRIII Protein, FCR-10 Protein, FCRIII Protein, FCRIIIA Protein, IGFR3 Protein, Fcgr4 Protein, Fc fragment of IgG receptor IIIa Protein, FCGR3A Protein, Fcgr3a Protein

Background: CD16A, FCGR3A, FCGRIIIA, FCR3A, FCRIIIA, IGFR3A, IGFRIIIA
Background: Low affinity immunoglobulin gamma Fc receptor IIIa, also known as FcγRIIIa or CD16a, is a type I integral membrane glycoprotein. CD16a is a member of the immunoglobulin superfamily and is expressed on macrophages, monocytes and NK cells. CD16a binds monomeric IgG with low affinity but is efficient at binding immune complexes and functions in NK cell activation, phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). CD16a is structurally composed of two extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail. CD16a is associated with a dimer of the common Fc receptor gamma-chain which contains the immunoreceptor tyrosine-based activation (ITAM) motif. The product provided only contains the extracellular portion of CD16a. CD16a has two allotypic variants differing at amino acid position 176, one containing phenylalanine (F176) and the other valine (V176). The V176 variant has a greater affinity for all the IgG subclasses and thus results in greater effector function.

Molecular Weight: 26.3 kDa

UniProt: P08637