Fibronectin Protein
Quick Overview for Fibronectin Protein (ABIN7318495)
Target
See all Fibronectin ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Human Fibronectin/FN Protein (Active)
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Sequence
- Pro1270-Ser1546&Ala1721-Thr2016
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Characteristics
- Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml.
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Fibronectin (AA 1087-1172) protein (His tag)
Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibronectin (AA 1008-1231) protein (His-IF2DI Tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25 % Sucrose, pH 6.2.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Fibronectin
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Alternative Name
- Fibronectin/FN
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Background
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Background: Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.
Synonym: Fibronectin, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
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Molecular Weight
- 62.7 kDa
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UniProt
- P02751
Target
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