GRK6 Protein (GST tag,His tag)
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- Target See all GRK6 Proteins
- GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This GRK6 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Human GRK6/GPRK6 Protein (His & GST Tag)(Active)
- Sequence
- Met 1-Arg 589
- Characteristics
- A DNA sequence encoding the human GRK6 isofrom B (P43250-2) (Met 1-Arg 589) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 93 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 7 nmol/min/mg using casein as substrate.
- Top Product
- Discover our top product GRK6 Protein
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 0.5 mM PMSF, pH 7.4
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- GRK6 (G Protein-Coupled Receptor Kinase 6 (GRK6))
- Alternative Name
- GRK6/GPRK6 (GRK6 Products)
- Synonyms
- MGC83187 Protein, GRK6 Protein, GPRK6 Protein, Gprk6 Protein, G protein-coupled receptor kinase 6 Protein, G protein-coupled receptor kinase 6 S homeolog Protein, GRK6 Protein, grk6.S Protein, grk6 Protein, Grk6 Protein
- Background
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Background: G protein-coupled receptor kinase 6, also known as G protein-coupled receptor kinase GRK6, GRK6 and GPRK6, is a lipid-anchor protein which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRK6 / GPRK6 contains one AGC-kinase C-terminal domain, one protein kinase domain and one RGS domain. This protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described. GRK6 / GPRK6 is widely expressed. GRK6 / GPRK6 silencing causes suppression of signal transducer and activator of transcription 3 (STAT3) phosphorylation associated with reduction in MCL1 levels and phosphorylation, illustrating a potent mechanism for the cytotoxicity of GRK6 inhibition in multiple myeloma (MM) tumor cells. GRK6 also appears to be involved in responses to morphine. Inhibition of GRK6 represents a uniquely targeted novel therapeutic strategy in human multiple myeloma.
Synonym: GPRK6
- Molecular Weight
- 95.1 kDa
- Pathways
- Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Negative Regulation of Transporter Activity
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