GFRA3 Protein (Fc Tag)

Catalog No. ABIN7320117

This Recombinant GFRA3 protein is expressed in HEK-293 Cells.

Quick Overview for GFRA3 Protein (Fc Tag) (ABIN7320117)

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 80 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This GFRA3 protein is labelled with Fc Tag.

Purpose: Recombinant Mouse GFRA3/GFR-alpha-3 Protein (Fc Tag)

Sequence: Met 1-Arg 379

Characteristics: A DNA sequence encoding the mouse GFRA3 (O35118) (Met 1-Arg 379), was fused with the Fc region of human IgG1 at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: GFRA3 (GDNF Family Receptor alpha 3 (GFRA3))

Alternative Name: GFRA3/GFR-alpha-3

Background:

Background: Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3 / GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor α3 (GFRA3 / GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons.

Synonym: GFRalpha3;Y15110

Molecular Weight: 66.2 kDa

UniProt: O35118