GNMT Protein (His tag)

Catalog No. ABIN7318543

Recombinant GNMT protein expressed in Escherichia coli (E. coli).

Quick Overview for GNMT Protein (His tag) (ABIN7318543)

Target: GNMT (Glycine N-Methyltransferase (GNMT))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This GNMT protein is labelled with His tag.

Purpose: Recombinant Human GNMT Protein (His Tag)

Sequence: Met 1-Asg294

Characteristics: Recombinant Human Glycine N-Methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asg294 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: GNMT (Glycine N-Methyltransferase (GNMT))

Alternative Name: GNMT

Background:

Background: Glycine N-Methyltransferase (GNMT) is a tetrameric cytosolic protein. GNMT catalyzes the synthesis of N-methylglycine from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. It can affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine, playing an important role in maintaining normal AdoMet levels. GNMT is highly expressed in liver. As a major folate-binding protein, GNMT takes part in the detoxification pathway. Defects in GNMT are the cause of hypermethioninemia. the patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.

Synonym: Glycine N-Methyltransferase, GNMT

Molecular Weight: 34.9 kDa

UniProt: Q14749

Pathways: Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process