GNMT Protein (AA 1-295) (His tag)
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- Target See all GNMT Proteins
- GNMT (Glycine N-Methyltransferase (GNMT))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-295
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GNMT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
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Glycine N-Methyltransferase (GNMT) (AA 1-100) protein (GST tag)
GNMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glycine N-Methyltransferase (GNMT) (AA 1-295) protein (His tag)GNMT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Glycine N-Methyltransferase (GNMT) (AA 1-295) protein (GST tag)GNMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glycine N-Methyltransferase (GNMT) (AA 2-295) protein (His tag)Crystallography grade GNMT Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycine N-Methyltransferase (GNMT) (AA 2-293) protein (His tag)Crystallography grade GNMT Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycine N-Methyltransferase (GNMT) protein (Myc-DYKDDDDK Tag)GNMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GNMT (Glycine N-Methyltransferase (GNMT))
- Alternative Name
- GNMT (GNMT Products)
- Synonyms
- glycine N-methyltransferase Protein, CpipJ_CPIJ015956 Protein, Gnmt Protein, GNMT Protein
- Background
- Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC). Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 34.9 kDa (315aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_061833
- UniProt
- Q14749
- Pathways
- Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
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