Glycoprotein A33 (Transmembrane) S Homeolog (GPA33.S) protein (His tag)

Catalog No. ABIN7195963

This Recombinant Glycoprotein A33 (Transmembrane) S Homeolog protein is expressed in HEK-293 Cells.

Quick Overview for Glycoprotein A33 (Transmembrane) S Homeolog (GPA33.S) protein (His tag) (ABIN7195963)

Target: Glycoprotein A33 (Transmembrane) S Homeolog (GPA33.S)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: His tag

Purpose: Recombinant Mouse GPA33/Glycoprotein A33 Protein (His Tag)

Sequence: Met 1-Ile 235

Characteristics: A DNA sequence encoding the mouse GPA33 (NP_067623.1) extracellular domain (Met 1-Ile 235) was expressed, with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Glycoprotein A33 (Transmembrane) S Homeolog (GPA33.S)

Alternative Name: GPA33/Glyco

Background:

Background: Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I  membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: 2010310L10Rik;2210401D16Rik;BB116197

Molecular Weight: 25.4 kDa

NCBI Accession: NP_067623