GLO1 Protein (His tag)

Catalog No. ABIN7320302

Recombinant GLO1 protein expressed in Escherichia coli (E. coli).

Quick Overview for GLO1 Protein (His tag) (ABIN7320302)

Target: GLO1 (Glyoxalase I (GLO1))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This GLO1 protein is labelled with His tag.

Purpose: Recombinant Mouse GLO1/Glyoxalase 1 Protein (His Tag)

Sequence: Ala 2-Ile 184

Characteristics: A DNA sequence encoding the mouse GLO1 (NP_079650.3) (Ala 2-Ile 184) was expressed, with a polyhistide tag at the N-terminus.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: GLO1 (Glyoxalase I (GLO1))

Alternative Name: GLO1/Glyoxalase 1

Background:

Background: Lactoylglutathione lyase, also known as Methylglyoxalase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, S-D-lactoylglutathione methylglyoxal lyase and GLO1, is a member of the glyoxalase I family. GLO1 / Glyoxalase I is a ubiquitous cellular defense enzyme involved in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Accumulative evidence suggests an important role of GLO1 expression in protection against methylglyoxal-dependent protein adduction and cellular damage associated with diabetes, cancer, and chronological aging. GLO1 / Glyoxalase I has been implicated in anxiety-like behavior in mice and in multiple psychiatric diseases in humans. GLO1 / Glyoxalase I catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. GLO1 / Glyoxalase I exists in three separable isoforms which originate from two alleles in the genome. These correspond to two homodimers and one heterodimer composed of two subunits showing different electrophoretic properties. GLO1 upregulation may play a functional role in glycolytic adaptations of cancer cells.

Synonym: 0610009E22Rik,1110008E19Rik,2510049H23Rik,AW550643,Glo-1,Glo-1r,Glo-1s,Glo1-r,Glo1-s,GLY1,Qglo

Molecular Weight: 21.6 kDa

NCBI Accession: NP_079650