Hemopexin Protein (HPX) (His tag)

Catalog No. ABIN7320830

Recombinant Hemopexin protein expressed in Human Cells.

Quick Overview for Hemopexin Protein (HPX) (His tag) (ABIN7320830)

Target: Hemopexin (HPX)

Protein Type: Recombinant

Origin: Mouse

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Hemopexin protein is labelled with His tag.

Purpose: Recombinant Mouse Hemopexin/HPX Protein (His Tag)

Sequence: Ser24-Gln460

Characteristics: Recombinant Mouse Hemopexin is produced by our Mammalian expression system and the target gene encoding Ser24-Gln460 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: Hemopexin (HPX)

Alternative Name: Hemopexin/HPX

Background:

Background: Hemopexin (HPX) is plasma glycoprotein belongs to the family of the acute-phase proteins whose synthesis is induced after an inflammatory event. Hemopexin with two four-bladed beta -propeller folds has been found in other proteins including collagenases and provides sites for protein-protein interactions. The liver is the major synthesizing organ. Hemopexin participates in maintaining and recycling the iron pool by utilizing its high binding affinity toward heme composed of protoporphyrin IX and iron. It also functions in preventing oxidation caused by heme after hemolysis. Hydrophobic heme molecules can intercalate into lipid membranes and participate in the oxidation of lipid membrane components through the Fenton reaction resulting in lipid peroxidation. Hemopexin undergoes a conformational change upon the binding of heme. The conformational change allows hemopexin to interact with a specific receptor, forming a complex which is then internalized. Heme concentrations in plasma increase after hemolysis, which is associated with several pathological conditions such as reperfusion injury and ischemia.

Synonym: Hemopexin,Hpx,Hpxn

Molecular Weight: 49.8 kDa

UniProt: Q91X72

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response