TIM3 Protein (His tag)

Catalog No. ABIN7319446

This Recombinant TIM3 protein is produced in Human Cells.

Quick Overview for TIM3 Protein (His tag) (ABIN7319446)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This TIM3 protein is labelled with His tag.

Purpose: Recombinant Human TIM-3/HAVCR2 Protein (His Tag)(Active)

Sequence: Ser22-Arg200 (Arg140Leu)

Characteristics: Recombinant Human TIM-3 is produced by our Mammalian expression system and the target gene encoding Ser22-Arg200 (Arg140Leu) is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human Galectin-9-GST at 2μg/ml(100 μl/well) can bind Human TIM-3-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: TIM-3/HAVCR2

Target Type: Virus

Background:

Background: T-Cell Membrane Protein 3 (TIM3) is a single-pass type I membrane protein that belongs to the TIM family of immunoglobulin superfamily. TIM3 includes a signal sequence (aa 1-21), an extracellular region (aa 22-202) with one Ig-like V-type domain, a transmembrane segment (aa 203-223), and a cytoplasmic domain (aa 224 - 301). TIM3 regulates macrophage activation, inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. It may be also involved in T-cell homing and as a receptor for LGALS9.

Synonym: Hepatitis A Virus Cellular Receptor 2, HAVcr-2, T-Cell Immunoglobulin and Mucin Domain-Containing Protein 3, TIMD-3, T-Cell Membrane Protein 3, TIM-3, HAVCR2, TIM3, TIMD3

Molecular Weight: 20.86 kDa

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints