HK3 Protein (GST tag,His tag)
Quick Overview for HK3 Protein (GST tag,His tag) (ABIN7196087)
Target
See all HK3 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This HK3 protein is labelled with GST tag,His tag.
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Purpose
- Recombinant Human Hexokinase-3/HK3 Protein (His & GST Tag)
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Sequence
- Met 1-Val 923
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Characteristics
- A DNA sequence encoding the human HK3 (P52790) (Met 1-Val 923) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Hexokinase 3 (White Cell) (HK3) (AA 1-922) protein (His tag)
custom-made HK3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Hexokinase 3 (White Cell) (HK3) (AA 1-923) protein (His tag)custom-made HK3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Hexokinase 3 (White Cell) (HK3) (AA 1-61) protein (His-IF2DI Tag)HK3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hexokinase 3 (White Cell) (HK3) (AA 1-923) protein (GST tag)HK3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Hexokinase 3 (White Cell) (HK3) protein (Myc-DYKDDDDK Tag)HK3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 7.4
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- HK3 (Hexokinase 3 (White Cell) (HK3))
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Alternative Name
- Hexokinase-3/HK3
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Background
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Background: Hexokinase-3, also known as Hexokinase type III, HKIII and HK3, is a protein which belongs to the hexokinase family. Hexokinase-3 / HK3 is an enzyme which in humans is encoded by the HK2 gene. Hexokinases phosphorylate glucose to produce glucose 6-phosphate, committing glucose to the glycolytic pathway. In mammalian tissues hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, with the exception of hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, this allosteric enzyme is inhibited by its product glucose 6-phosphate.
Synonym: HKIII;HXK3
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Molecular Weight
- 127 kDa
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UniProt
- P52790
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Pathways
- Carbohydrate Homeostasis, Warburg Effect
Target
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