HPRT1 Protein (His tag)

Catalog No. ABIN7318580

This Recombinant HPRT1 protein is produced in Escherichia coli (E. coli).

Quick Overview for HPRT1 Protein (His tag) (ABIN7318580)

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This HPRT1 protein is labelled with His tag.

Purpose: Recombinant Human HGPRT/HPRT1 Protein (His Tag)

Sequence: Met 1-Ala218

Characteristics: Recombinant Human Hypoxanthine-Guanine Phosphoribosyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Ala218 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 250 mM NaCl, 2 mM EDTA, 30 % Glycerol, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: HGPRT/HPRT1

Background:

Background: Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT) has an important role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 functions to salvage purines from degraded DNA to renewed purine synthesis, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.

Synonym: Hypoxanthine-Guanine Phosphoribosyltransferase, HGPRT, HGPRTase, HPRT1, HPRT

Molecular Weight: 27.8 kDa

UniProt: P00492

Pathways: Ribonucleoside Biosynthetic Process