IDE Protein (His tag)
Quick Overview for IDE Protein (His tag) (ABIN7318623)
Target
See all IDE ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This IDE protein is labelled with His tag.
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Purpose
- Recombinant Human IDE/Insulysin Protein (His Tag)
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Sequence
- Met42-Leu1019
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Characteristics
- Recombinant Human Insulin-Degrading Enzyme is produced by our Mammalian expression system and the target gene encoding Met42-Leu1019 is expressed with a 6His tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Insulin-Degrading Enzyme (IDE) (AA 1-1019) protein (His tag)
custom-made IDE Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Insulin-Degrading Enzyme (IDE) (AA 1-1019) protein (His tag)custom-made IDE Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Insulin-Degrading Enzyme (IDE) (AA 920-1019) protein (GST tag)IDE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Insulin-Degrading Enzyme (IDE) (AA 1-1019) protein (GST tag)IDE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Insulin-Degrading Enzyme (IDE) protein (Myc-DYKDDDDK Tag)IDE Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,0.05 % Brij35,10 %Glycerol, pH 7.5.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- IDE (Insulin-Degrading Enzyme (IDE))
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Alternative Name
- IDE/Insulysin
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Background
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Background: Insulin-Degrading Enzyme (IDE) is a secreted enzyme that belongs to the peptidase M16 family. IDE is a large zinc-binding protease and cleaves multiple short polypeptides that vary considerably in sequence. IDE plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. IDE degrades amyloid formed by APP and IAPP. IDE may participate in the degradation and clearance of naturally secreted amyloid β-protein by neurons and microglia. IDE, which migrates at 110 kDa during gel electrophoresis under denaturing conditions, has since been shown to have additional substrates, including the signaling peptides glucagon, TGF α and β-endorphin.
Synonym: Insulin-Degrading Enzyme, Abeta-Degrading Protease, Insulin Protease, Insulinase, Insulysin, IDE
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Molecular Weight
- 114.3 kDa
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UniProt
- P14735
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Pathways
- SARS-CoV-2 Protein Interactome
Target
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