IL-25 Protein (Fc Tag)

Catalog No. ABIN7321197

This Recombinant IL-25 protein is expressed in HEK-293 Cells.

Quick Overview for IL-25 Protein (Fc Tag) (ABIN7321197)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 85 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This IL-25 protein is labelled with Fc Tag.

Purpose: Recombinant Rat Interleukin-25/IL-25 Protein (Fc Tag)(Active)

Sequence: Val17-Ala169

Characteristics: A DNA sequence encoding the rat IL25 (D3ZLB1) (Val17-Ala169) was expressed, fused with Fc region of human IgG1 at the N-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized mouse IL17BR-His at 10 μg/mL (100 μl/well) can bind rat Fc-IL25. The EC50 of rat Fc-IL25 is 0.12-0.27 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: Interleukin-25/IL-25

Background:

Background: Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)–activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.

Synonym: IL25

Molecular Weight: 46.1 kDa

UniProt: D3ZLB1