IL-25 Protein (AA 33-177) (His tag)
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- Target See all IL-25 (IL25) Proteins
- IL-25 (IL25) (Interleukin 25 (IL25))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 33-177
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL-25 protein is labelled with His tag.
- Purpose
- Human IL-25/IL-17E Protein
- Sequence
- Tyr33-Gly177
- Characteristics
- Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 25 (IL25) (AA 33-177) protein (His-Avi Tag,Biotin)
IL25 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (Active) proteinIL25 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 25 (IL25) (AA 17-169) protein (His tag)IL25 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 1-153) protein (His tag)IL25 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 25 (IL25) (AA 33-177) protein (His tag)IL25 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 1-177) protein (GST tag)IL25 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 25 (IL25) (Active) protein (Fc Tag)IL25 Origin: Rat Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
Interleukin 25 (IL25) proteinIL25 Origin: Human Host: Escherichia coli (E. coli) Recombinant
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-25 (IL25) (Interleukin 25 (IL25))
- Alternative Name
- IL-25 (IL25 Products)
- Synonyms
- IL17E Protein, IL-17e Protein, Il17e Protein, IL25 Protein, RGD1561632 Protein, interleukin 25 Protein, IL25 Protein, Il25 Protein
- Background
- IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
- Molecular Weight
- 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
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