IL4 Receptor Protein (His tag)

Catalog No. ABIN7320981

Product Details

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: Human Cells

Purification tag / Conjugate: This IL4 Receptor protein is labelled with His tag.

Purpose: Recombinant Cynomolgus Interleukin-4 Receptor Subunit Alpha/IL-4 Rα Protein (His Tag)

Sequence: Met26-Arg232

Characteristics: Recombinant Cynomolgus IL-4 Rα is produced by our Mammalian expression system and the target gene encoding Met26-Arg232 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human IL-4(Cat: PKSH033456) at 10μg/ml(100 μl/well) can bind Human IL-4Ra-His. The ED50 of Human IL-4Ra-His is 7μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: Interleukin-4 Receptor Subunit Alpha/IL-4 Ralpha (IL4R Products)

Background:

Background: Interleukin-4 receptor subunit alpha(IL-4RA), alos known as Soluble IL-4 receptor subunit alpha, belongs to the type I cytokine receptor family and type 4 subfamily. It expressed in both Th1 and Th2 cells. It functions as receptor for both interleukin 4 and interleukin 13 and couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and chemokine and mucus production at sites of allergic inflammation. In certain cell types, IL-4RA can signal through activation of insulin receptor substrates, IRS1/IRS2. The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequently it recruits to the complex of the common gamma chain. In immune cells, IL-4RA creates a type I receptor. In non-immune cells, it forms a type II receptor with of IL13RA1. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor and interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP.

Synonym: Interleukin-4 receptor subunit alpha,IL-4R-alpha,CD124,IL4-BP,Soluble IL-4R-alpha

Molecular Weight: 24.6 kDa

UniProt: G7Q0S7

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process