IL4 Receptor Protein (AA 24-232) (His tag)

Catalog No. ABIN7274992

Product Details

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Protein Type: Recombinant

Protein Characteristics: AA 24-232

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL4 Receptor protein is labelled with His tag.

Purpose: Human IL-4 R alpha/CD124 Protein

Sequence: Gly24-His232

Characteristics: Recombinant Human IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-His232.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-4 R alpha, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 11.76ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: IL-4 R alpha (IL4R Products)

Synonyms: IL4R Protein, il-4ra Protein, zgc:113063 Protein, CD124 Protein, IL-4RA Protein, IL4RA Protein, Il4ra Protein, IL4 Protein, Il4r Protein, interleukin 4 receptor Protein, interleukin 4 receptor, tandem duplicate 1 Protein, interleukin 4 receptor, alpha Protein, IL4R Protein, il4r.1 Protein, Il4r Protein, Il4ra Protein

Background: Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family.The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.

Molecular Weight: 24.7 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process