KIRREL Protein (His tag)

Catalog No. ABIN7320809

This Recombinant KIRREL protein is produced in Human Cells.

Quick Overview for KIRREL Protein (His tag) (ABIN7320809)

Target: KIRREL (NEPH1) (Kin of IRRE Like 1 (NEPH1))

Protein Type: Recombinant

Origin: Mouse

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This KIRREL protein is labelled with His tag.

Purpose: Recombinant Mouse KIRREL1/NEPH1 Protein (His Tag)

Sequence: Leu48-Leu525

Characteristics: Recombinant Mouse KIRREL1 is produced by our Mammalian expression system and the target gene encoding Leu48-Leu525 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: KIRREL (NEPH1) (Kin of IRRE Like 1 (NEPH1))

Alternative Name: KIRREL1/NEPH1

Background:

Background: Kin of irregular chiasm-like protein 1(Kirrel1), also known as Nephrin-like protein 1(Neph1), belongs to the immunoglobulin superfamily. Kirrel1 plays a significant role in the normal development and function of the glomerular permeability. It is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. The knockout of this gene could result in perinatal lethality accompanied by proteinuria, and effacement of glomerular podocytes. Kirrel1 is abundantly expressed in kidney and specifically expressed in podocytes of kidney glomeruli. Its' subunit interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus) and interacts with NPHS1/nephrin (via the Ig-like domains). This interaction is dependent on KIRREL glycosylation. Kirrel1 also interacts when tyrosine-phosphorylated with GRB2.

Synonym: Kin of IRRE-like protein 1,Kin of irregular chiasm-like protein 1,Nephrin-like protein 1,Kirrel1,Neph1

Molecular Weight: 53.4 kDa

UniProt: Q80W68

Pathways: Regulation of Actin Filament Polymerization