LGMN Protein (His tag)

Catalog No. ABIN7318718

Recombinant LGMN protein expressed in Human Cells.

Quick Overview for LGMN Protein (His tag) (ABIN7318718)

Target: LGMN (Legumain (LGMN))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This LGMN protein is labelled with His tag.

Purpose: Recombinant Human Legumain/LGMN Protein (His Tag)

Sequence: Ile18-Tyr433

Characteristics: Recombinant Human Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Ile18-Tyr433 is expressed with a 6His tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10 % Glycerol, pH 8.0.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: LGMN (Legumain (LGMN))

Alternative Name: Legumain/LGMN

Background:

Background: Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.

Synonym: Legumain, Asparaginyl Endopeptidase, Protease Cysteine 1, LGMN, PRSC1

Molecular Weight: 48.7 kDa

UniProt: Q99538

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades