Quick Overview for LGMN Protein (His tag) (ABIN7320693)
Target
See all LGMN ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This LGMN protein is labelled with His tag.
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Purpose
- Recombinant Mouse Legumain/LGMN Protein (His Tag)
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Sequence
- Val 18-Tyr 435
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Characteristics
- Recombinant Mouse Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Val18-Tyr435 is expressed with a 6His tag at the C-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Legumain (LGMN) (AA 18-433) protein (His tag)
LGMN Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Legumain (LGMN) (AA 1-433) protein (GST tag)LGMN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Legumain (LGMN) (Active) protein (His tag)LGMN Origin: Mouse Host: HEK-293 Cells Recombinant > 75 % as determined by SDS-PAGE Active
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Restrictions
- For Research Use only
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Format
- Frozen, Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl,20 % Glycerol, pH 8.0.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- LGMN (Legumain (LGMN))
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Alternative Name
- Legumain/LGMN
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Background
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Background: Mouse Legumain,also known as LGMN, is a cysteine protease belonging to peptidase family C13 and is expressed in kidney and placenta abundantly. LGMN has a strict specificity for hydrolysis of asparaginyl bonds. It can also cleave aspartyl bonds slowly, especially under acidic conditions. The mammalian legumain is involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. It plays a role in the regulation of cell proliferation via its role in EGFR degradation. Legumain deficiency causes the accumulation of pro-Cathepsins B, H and L, another group of lysosomal cysteine proteases. Overexpression of Legumain in tumors is significant for invasion/metastasis. Mammalian legumain is inhibited by iodoacetamide and maleimides. Legumain activation appears to be autocatalytic and can be triggered by acidic pH .
Synonym: Legumain,Lgmn,Asparaginyl endopeptidase,Protease cysteine 1,Prsc1,AEP
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Molecular Weight
- 48.7 kDa
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NCBI Accession
- NP_035305
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Pathways
- Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades
Target
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